Study of growth condition of medicinal plants under different water stress levels could be as an indicator for cultivation of drought resistance species in water scarce regions. Abiotic stresses such as drought stress are the main limitation factors in crop production and landscape development in arid or semi-arid regions such as Iran. Using drought resistant medicinal plants for developing landscape is the best solution. This study was conducted to evaluate the Cichorium intybus under four water stress levels including 45, 60, 75 and 90% field capacity in three replications over 2014-2015 in Isfahan University nursery. Results showed significant effects (P<0.01) on all morphological and physiological characteristics. Proline and anthocyanin content of leaf and flower, and carotenoid were increased but chlorophyll a, b and Chlorophyll total content were decreased by increasing water stress severity at 45% field capacity.  Plant biomass (fresh and dry weight) decreased by increasing water stress severity. Time to initiate flowering, full flowering stage, leaf length and number, flower number and flowering period were decreased by increasing water stress severity. Drought stress on proline and anthocyanin content of leaf and flower, and Chlorophyll a, b and Chlorophyll total and carotenoid was significant. Results showed that Cichorium intybus is a relatively drought tolerance plant suitable for urban landscapes.