Journal of Plant Process and Function

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Madadi M, Khomari S, Javadi A, Sofalian O. Effect of black cumin seed priming with calcium nitrate and nano-zinc oxide on germinability and seedling growth under salinity stress. Plant Process and Function 2016; 5 (15) :169-180
URL: http://jispp.iut.ac.ir/article-1-381-en.html

The varying seed priming methods have widely been used for the improving germinability and seedling establishment of numerous economic plants especially under environmental stresses. In this context, in order to evaluate the advantage of priming on different physiological and biochemical indices of black cumin seed germination and seedling growth in saline conditions, the factorial experiment was conducted based on RCB design with four replicates in the faculty of agricultural science laboratory complex. Experimental factors included five priming methods (non-priming control, priming with calcium nitrate, nano zinc oxide, calcium nitrate+nano zinc oxide and hydro-priming) and three salinity levels (0, 50 and 100 mM NaCl). The results of the present research indicated that despite the inhibitory effect of salinity on black cumin seed germination and seedling growth characteristics, seed pre-treatment substantially alleviated the deleterious impacts of salt due to enhancement of prescribed parameters. When NaCl concentration increased in germination medium, total protein content of seedling was reduced and catalase activity was upregulated, while seed priming caused increase of the both traits under salinity. Among the applied priming methods, seed pre-treatment with calcium nitrate+nano zinc oxide had the highest efficacy and the best performance in ameliorating inhibition of measured parameters of black cumin induced by salt.