Volume 15, Issue 72 (vol. 15, no. 72 2026)
2026, 15(72): 193-206 |
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Rastgoo A, Pourbeyrami Hir Y, Chamani E, Mirjalili M H, Miran M. Investigation of cell growth dynamics, activities of phenylpropanoid pathway enzymes, and rosmarinic acid accumulation in cell suspension cultures of
Salvia leriifolia Benth.. Plant Process and Function 2026; 15 (72) : 12
URL: http://jispp.iut.ac.ir/article-1-2276-en.html
URL: http://jispp.iut.ac.ir/article-1-2276-en.html
Arefeh Rastgoo1 
, Younes Pourbeyrami Hir *2 , Esmaeil Chamani1 , Mohammad Hossein Mirjalili3 , Mansour Miran4
, Younes Pourbeyrami Hir *2 , Esmaeil Chamani1 , Mohammad Hossein Mirjalili3 , Mansour Miran4
1- Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2- Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran ,*Younes_ph62@uma.ac.ir
3- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, 1983969411, Tehran, Iran
4- Department of Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
2- Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran ,
3- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, 1983969411, Tehran, Iran
4- Department of Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
Abstract: (31 Views)
Species of the genus Salvia, including Salvia leriifolia Benth., are recognized as important sources of phenolic acids, which have generated increasing interest in enhancing their production through plant tissue and cell culture technologies. This study aimed to characterize biomass accumulation, key enzymatic activities, and metabolite biosynthesis during the cell growth cycle of S. leriifolia. Leaf explants were used to establish cell suspension cultures, and a medium containing 15 µM BA and 20 µM NAA was identified as the optimal hormone treatment for promoting cell proliferation. Over a five-week culture period, cell biomass, total phenolics, total flavonoids, antioxidant activity (DPPH, IC₅₀), PAL and TAT enzyme activities, and rosmarinic acid content were assessed at weekly intervals. The experiment was conducted in a completely randomized design, and data were analyzed using analysis of variance followed by mean comparison tests. Significant differences were observed among treatments at p < 0.05 and p < 0.01. The results indicated that the growth curve of the suspension cells followed a typical sigmoidal pattern, with the highest biomass observed at the end of the fourth week (Day 28). Cell viability gradually declined from approximately 98% in the first week (day 7) to nearly 80% by the fifth week (day 35). The maximum levels of total phenolics, total flavonoids, and antioxidant activity were recorded in the third week (day 21). Similarly, total protein content, as well as peak PAL and TAT activities during the third and fourth weeks (days 21 and 28), coincided with the highest accumulation of rosmarinic acid (42.69 mg g⁻¹ DW). Overall, these findings provide a solid basis for optimizing rosmarinic acid production in plant cell culture systems through targeted hormonal regulation, improved culture conditions, and supplementation with biosynthetic pathway precursors.
Article number: 12
Type of Study: Research |
Subject:
biotechnology
Received: 2025/11/17 | Accepted: 2026/02/10 | Published: 2026/06/2
Received: 2025/11/17 | Accepted: 2026/02/10 | Published: 2026/06/2
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