Rahmati Dehkordi M, Dehestani-Ardakani M, Kamali Aliabad K, Jafari A. Evaluation of the effect of silver nanoparticles and chemical disinfectants on reducing contamination and plant growth regulators on callus induction in anthurium (Anthurium andreanum Lindl.) plant. Plant Process and Function 2025; 14 (69) : 15
URL:
http://jispp.iut.ac.ir/article-1-2209-en.html
1- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran
2- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran & Medicinal and Industrial Plant Research Institute, Ardakan, Iran , mdehestani@ardakan.ac.ir
3- Department of Soil Science, Faculty of Agriculture & Natural Resources, Yazd University, Yazd
Abstract: (26 Views)
In the present study, the micropropagation of Anthurium (Anthurium andraeanum Lindl.) was investigated in the form of two separate experiments. In the first experiment, the effect of different types of chemical disinfectants on the reduction of contamination in leaf and bud explants was tested in a factorial experiment within a completely randomized design with three replicates. The first factor included six different disinfection methods (S1, S2, S3, S4, S5, and S6), the second factor comprised two types of explants (leaf and bud), and the third factor included two cultivars of Anthurium (red and lipstick). The results showed that the lowest contamination rate (zero percent) was achieved in treatment S6 (silver nanoparticles) for the red cultivar and leaf explant, as well as in treatments S2 and S6 for the lipstick cultivar and leaf explant, and also in treatment S1 for the bud explant of the same cultivar. In the second experiment, the effect of plant growth regulators on callus induction in leaf explants of the “lipstick cultivar was investigated in a factorial design within a completely randomized design with three replicates. The first factor comprised 11 treatments (T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, and T11), and the second factor included two types of culture media (MS and ½ MS). The highest callus formation was observed in T3 treatment (2 mg L-1 BA + 0.5 mg L-1 2,4-D + 5% coconut water), at a rate of 40.00%. For regeneration, the calluses were transferred to the MS medium containing 1 mg L-1 2,4-D + 0.5 mg L-1 NAA + 1.5 mg L-1 BA. Overall, the use of compounds such as silver nanoparticles and antibiotics showed a positive effect in reducing contamination in the explants, and the ½ MS medium combined with BA and 2,4-D proved to be effective in callus induction in leaf explants.
Article number: 15
Type of Study:
Research |
Subject:
Horticulture Received: 2025/05/1 | Accepted: 2025/08/26 | Published: 2025/12/16
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