The effect of Mycorrhiza, Rhizobium and Azospirillum application on some physiological traits, antioxidant enzyme activity, and yield of two bean cultivars (Koosha chitti bean and Yaghut red bean) under different irrigation conditions

Ghaseme Pirbaloti, Ahmad; Rafieolhossaini, Mohammad; Ghobadinia, Mahdi; Salehi, Foroud

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Ghaseme Pirbaloti A, Rafieolhossaini M, Ghobadinia M, Salehi F. The effect of Mycorrhiza, Rhizobium and Azospirillum application on some physiological traits, antioxidant enzyme activity, and yield of two bean cultivars (Koosha chitti bean and Yaghut red bean) under different irrigation conditions. Plant Process and Function 2025; 14 (69) : 6
URL: http://jispp.iut.ac.ir/article-1-2191-en.html

The effect of Mycorrhiza, Rhizobium and Azospirillum application on some physiological traits, antioxidant enzyme activity, and yield of two bean cultivars (Koosha chitti bean and Yaghut red bean) under different irrigation conditions

Ahmad Ghaseme Pirbaloti¹

, Mohammad Rafieolhossaini ^*¹

, Mahdi Ghobadinia²

, Foroud Salehi³

1- Agrronomy Department, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran
2- Department of Water Engineering, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran
3- Field and Horticulture Sciences Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, Isfahan, Iran

Abstract: (29 Views)

Drought is one of the factors limiting the yield and growth of plants. Beans are one of the most important legumes for food security and human consumption. Using biofertilizers can increase the plants’ tolerance to abiotic stress. In order to evaluate the effect of biofertilizers on physiological traits and activity of some antioxidant enzymes of bean genotypes under deficit irrigation, an experiment was conducted as a split plot factorial based on a randomized complete block design with three replications at Shahrekord University Research Farm in 2024. Experimental treatments included irrigation regimes with three levels as main plots, including irrigation at 100% and deficit irrigation at 80% and 60% of beans' water requirement. The sub-factorial factor was biological fertilizer with four levels as control: Rhizobium leguminosarum, Azosprillium brasilese, and Arbuscular Mycorrhiza. The two common bean cultivars used included red bean and chiti cultivars. The findings of this research indicated that the use of biofertilizers caused a significant increase in chlorophyll a, b and total chlorophyll at all irrigation levels compared to the treatment without fertilizers. The effect of biofertilizers on the activity of antioxidant enzymes showed that mycorrhiza and rhizobium at 80% of water requirement caused a significant increase of catalase (CAT) in red beans. The highest activity of ascorbate peroxidase (APX) was obtained in 80% water requirement × Rhizobium biofertilizer in red beans. Guaiacol peroxidase (GPX) had the highest activity in pinto beans at 80% of water requirement with the use of Azosprillium fertilizer. In general, according to the interaction effects of irrigation levels and the use of biofertilizers, red beans had higher enzymatic activity compared to pinto beans in most treatments. The amount of soluble protein decreased with increasing deficit irrigation. However, among the biofertilizers, only Rhizobium was able to increase the amount of this trait compared to the control. The highest concentration of hydrogen peroxide was obtained under deficit irrigation conditions of 60% of water requirement. The lowest amount of hydrogen peroxide was observed in the application of mycorrhiza biofertilizer, which was significantly different from other biofertilizers. Mycorrhiza and rhizobium biofertilizers significantly increased the grain yield of bean cultivars under the conditions of 100 and 80% of water requirement, compared to the Azosprillium and control treatments. The results of this study showed that the application of biofertilizers under deficit irrigation conditions can improve the synthesis of photosynthetic pigments and the activity of antioxidant enzymes and increase bean seed yield.

Article number: 6

Keywords: Deficit irrigation, Mycorrhiza, Rhizobium, Azospirillum, Chlorophyll, Catalase (CAT), Ascorbate Peroxidase (APX), Guaiacol Peroxidase (GPX)

Full-Text [PDF 1431 kb] (31 Downloads)

Type of Study: Research | Subject: Droughts Stress
Received: 2025/03/9 | Accepted: 2025/06/10 | Published: 2025/12/16