جلد 14، شماره 70 - ( جلد 14، شماره 70، بهمن و اسفند 1404 )                   جلد 14 شماره 70 صفحات 10-1 | برگشت به فهرست نسخه ها

‎ DOI: 10.22034/14.70.1

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Effect of Pb and Cd on the response of some stress genes and the activity of antioxidant enzymes in date palm leaves
Muna Mohammed Najeeb1 ، Rashid Jamei*2 ، Jalil Khara1 ، Aqeel Hadi Abdul Wahid3
1- Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2- Department of Biology, Faculty of Science, Urmia University, Urmia, Iran ، r.jamei@urmia.ac.ir
3- Department of Horticulture, College of Agriculture, University of Basrah, Iraq
چکیده:   (344 مشاهده)
This study used 18-month-old palm offshoots irrigated with water containing different concentrations of heavy metals: Pb at 100 and 200 mg/kg soil, and Cd at 3 and 6 mg/kg soil. After three months of treatment, a sample of palm leaves was taken for molecular and chemical tests. The results showed that treatment with Pb and Cd increased their concentrations compared with the control sample, especially at high concentrations. This treatment caused stress to the plants and affected their biochemical and molecular characteristics. The content of H2O2 increased significantly in the treatment with 200 mg/kg Pb, reaching the highest level of 1.18±2.67 μmol/g. In contrast, the control sample had the lowest H2O2 content (0.90 ± 0.01 μmol/g). Peroxidase activity also increased significantly during treatment, with the highest enzyme activity (40.49 ± 0.26 units/min/g) observed in the Pb 200 mg/kg group. Treatment with Pb at 200 mg/kg also resulted in the highest soluble carbohydrate content (15.25 ± 0.72 mg/g) compared with the control sample (8.63 ± 0.44 mg/g). Photosynthetic pigments, including chlorophylls and carotenoids, were significantly reduced, especially at high treatment concentrations. Treatment with Pb at 200 mg/kg led to decreases in chlorophyll a and b contents (2.17±0.13 and 1.14±0.07 mg/g Fw, respectively) compared with the control (4.14±0.07 and 1.47±0.11 mg/g Fw, respectively). Similarly, Cd treatment at 3 and 6 mg/kg caused decreases in chlorophyll a and b contents compared with the control sample. The content of carotenoids also decreased significantly (1.02 ± 0.03 and 1.16 ± 0.08 mg/g FW, respectively) when treated with high concentrations of Pb and Cd compared with the control sample (1.87 ± 0.14 mg/g FW). Regarding the effect of heavy metal application on gene expression levels of threonine specific kinase/serine (salt tolerance kinases) (STK), phosphatidylinositol 4,5-bisphosphate 2 (PIP2), pathogenesis-related protein 1 (PR1), and superoxide dismutase (SOD) genes, treatments with Pb at 100 and 200 mg/kg resulted in the highest expression of the PIP2 gene (3.39±0.46 and 4.81±0.30-fold, respectively) compared with the control sample.
شماره‌ی مقاله: 1
واژه‌های کلیدی: Antioxidant enzymes، Cadmium، Date palm، Gene expression، Lead
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نوع مطالعه: پژوهشي | موضوع مقاله: تنش فلزات سنگین
دریافت: 1403/3/26 | پذیرش: 1403/7/3 | انتشار: 1404/12/9
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