Volume 13, Issue 60 (vol. 13, no. 60 2024)                   2024, 13(60): 223-234 | Back to browse issues page

‎ DOI: 10.22034/13.60.223

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Evaluation of the effect of culture medium and different hormonal compounds on in vitro parameters during the micropropagation of vanilla orchid (Vanilla planifolia)
Yusuf Farrokhzad1 , Mahnaz Salatin2 , Shahram Jafarnia * 3
1- Center for Advanced Research and Development of Elite Affairs, ETKA Organization, Tehran, Iran
2- Medicinal Plants Section, Iranian Modern Farms Holding, Tehran, Iran
3- Medicinal Plants Section, Iranian Modern Farms Holding, Tehran, Iran , sh.jafarnya@gmail.com
Abstract:   (280 Views)
In this study, an efficient and simple method was developed for the micropropagation of vanilla orchid (Vanilla planifolia), which is a valuable spice-ornamental plant. The internodal explants after the disinfection process were established on eight culture mediums, including MS + 2 mg/L TDZ; MS + 2 mg/L TDZ + 0.5 mg/L NAA; MS + 2 mg/L Kin; MS + 2 mg/L Kin + 0.5 mg/L NAA; 1.2MS + 2 mg/L TDZ; 1.2MS + 2 mg/L TDZ + 0.5 mg/L NAA; 1.2MS + 2 mg per liter Kin; 1.2 MS + 2 mg/l Kin + 0.5 mg/L NAA. The treatments were arranged in the form of a completely randomized design (CRD) in three replications, followed by subculturing once every month. After three months and the completion of the shoot proliferation stage, different in vitro traits were evaluated. The results showed that the highest survival rates of explants and shoot multiplication occur on 1/2MS+2 mg/l TDZ+0.5 mg/l NAA and 1/2MS+2 mg/l Kin+0.5 mg/l NAA mediums. However, 1/2MS+2 mg/l TDZ+0.5 mg/l NAA medium resulted in the highest leaf number, fresh weight and percentage of dry weight. The length of tissue-cultured plants on MS+2 mg/l TDZ+0.5 mg/l NAA was greater than other mediums. The highest number of roots and root fresh weight were also observed at 1/2MS+2 mg/l TDZ+0.5 mg/l NAA, although compared to other mediums with NAA there was not a significant difference. In general, based on the obtained results, medium 1/2MS+2 mg/l TDZ+0.5 mg/l NAA is recommended for vanilla micropropagation.
Article number: 13
Keywords: Vanilla orchid, Micropropagation, Plant growth regulators, Plant tissue culture
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Type of Study: Research | Subject: Horticulture
Received: 2023/10/21 | Accepted: 2023/12/4 | Published: 2024/06/18
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