In order to investigate the effect of biofertilizer on physiological characteristics and nutrient concentration in linseed under irrigation withholding at different growth stages, a farm experiment conducted as split plot base on randomized complete block design with three replication at research field of Yasouj University in 2017. Irrigation regimes (full irrigation, irrigation withholding at beginning of flowering until beginning of podding, irrigation withholding at beginning of podding to end of maturity) and bio-fertilizer application at four levels (mycorrhiza fungi, Phosphate Barvar2, mycorrhiza arbuscular fungi + Phosphate Barvar2 and non-application of bio-fertilizer) were considered as the main and the sub plots, respectively. The results showed that the highest concentration of phosphorus (8.27 g.kg^-1) and potassium (4.20 g.kg^-1) in the leaf was observed in the treatment of mycorrhiza bio-fertilizer. In both stages of flowering and seed filling, irrigation withholding compared to full irrigation increased the soluble sugars, malondialdehyde, but reduced the relative water content and soluble protein of leaf.  The highest leaf prolin cintent (11.63 µmol.g^-1) was observed in both biofertilizer combination treatments in condition of Irrigation withholding at beginning of podding to end of maturity. Application of both type of biofertilizer increased soluble sugar concentration, soluble protein and reduced malondialdehyde levels. The highest grain yield was observed from application of mycorrhiza+ phosphate Barvar2.
The results indicate that linseed is sensitive to drought stress in the encapsulation to ripening stage (58% reduction). This damage modified by application of biofertilizer trough improving the absorption of nutrients and changing the concentration of osmolites.

Keywords: Droght, Linseed, mycorrhiza, Nutrients, Proline

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