Volume 8, Issue 33 (vol. 8, no. 33, 2020 2019)
2019, 8(33): 203-211 |
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Kalantari A, Koobaz P, Rostami M, Fathi Ghare baba M, Mojtahedi N. The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars. Plant Process and Function 2019; 8 (33) :203-211
URL: http://jispp.iut.ac.ir/article-1-1134-en.html
URL: http://jispp.iut.ac.ir/article-1-1134-en.html
Ashkaneh Kalantari1 
, Parisa Koobaz * 2, Majid Rostami1 , Mohammad Fathi Ghare baba3 , Narges Mojtahedi4
, Parisa Koobaz * 2, Majid Rostami1 , Mohammad Fathi Ghare baba3 , Narges Mojtahedi4
1- Department of Agriculture, Malayer University, Malayer, Iran
2- Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran ,pkoobaz@abrii.ac.ir
3- Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran
4- Department of Tissue culture, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran
2- Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran ,
3- Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran
4- Department of Tissue culture, Agricultural Biotechnology Research Institute of Iran, AREEO, Karaj, Iran
Abstract: (2582 Views)
Enhancement of in vitro micro corm production in Gladiolus as an alternative commercial method has been focus of attention. With respet to the position of Gladiolus in Iran's ornamental plants market and in order to provide the proper protocol for the proliferation of glycol corms by tissue culture method, the effect of different concentrations of naphthalene acetic acid (NAA) and benzyl aminopurine (BAP) in two explants and production of shoot and roots of two commercial gladiolus cultivars were investigated. In the next experiment the role of different concentrations of sucrose (in Murashige and Skoog plant growth liquid medium) in production of corms was studied and finally cold treatment in 3 times were used to break dormancy of propagated cormlets. All of the main experiments were conducted as factorial based on completely randomized design with 3 replications. The highest number of shoots (14) and roots (12) was observed in White cultivar with the treatments of 2mg/L BAP and 0.5 mg/ml NAA. Application of 60 g/L sucrose in MS media produced the highest number of cormlets (14) and also the largest cormlets (14.5 mm). Results of dormancy breaking experiment which performed at 4 °C for three periods showed that three months chilling at 4 °C was the best treatment to break dormancy in both cultivars.
Type of Study: Research |
Subject:
Plant Growth Regulators
Received: 2018/10/8 | Accepted: 2019/03/6 | Published: 2020/05/11
Received: 2018/10/8 | Accepted: 2019/03/6 | Published: 2020/05/11
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