Volume 8, Issue 32 (vol. 8, no. 32. 2020 2019)                   j.plant proc. func. 2019, 8(32): 311-324 | Back to browse issues page

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Akbari R, fahmideh L, Fazeli-Nasab3 B. Investigating the Possibility of Polyploidy Production and Its Effect on Phenolic Content and Antioxidant Enzymes of Carum copticum of Pakistani Population. j.plant proc. func.. 2019; 8 (32) :311-324
URL: http://jispp.iut.ac.ir/article-1-1047-en.html
1- , leila.fahmideh@yahoo.com
Abstract:   (1088 Views)
Nowadays, the induction chromosome doubling (polyploidization) using colchicine is regarded as one of the attractive methods for improving of medicinal plants in order to increase their production. In this research, colchicine was used for the induction of polyploidy and examining its effect on the amount of polyploidy and some morphological and physiological traits of Pakistani population of the Ajowan medical plant. Colchicine with concentrations of 0, 0.2, 0.5, 0.75 and 1 g/L, and for time durations of 6, 12 and 18 hours was used on the root apical meristem (RAM). A factorial experiment in completely randomized design (CRD) with three replications was used. Chromosomal analysis was carried out using squash technique and visual analysis method. The results showed that the number of somatic chromosomes in the examined species was either in diploid (2n = 2x = 18) or tetraploid (2n = 4x = 36) status. The highest percentage of polyploidy induction (80%) was obtained after dipping the seedlings at a concentration of 0.5 g/L colchicine for 6 hours. Then, after the treatment of the plant with colchicine and cultivating the treated and control seeds in the pot, the grown seedlings were studied at flowering stage. The results showed that tetraploid plants (treated) were superior to control group plants with regard to physiological (chlorophyll a, anthocyanin, ascorbate peroxidase, catalase and flavonoid) and morphological (number of lateral branches, plant height, number of leaves and fresh and dry weight) traits, therefore, the studied breeding method can be useful and applicable for the Ajwain (Trachyspermum ammi) plant.
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Type of Study: Research | Subject: biotecnology
Received: 2018/04/29 | Accepted: 2018/08/25 | Published: 2020/01/19

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