Plant Process and Function
فرآیند و کارکرد گیاهی
Journal of Plant Process and Function
Agriculture
http://jispp.iut.ac.ir
1
admin
2322-2727
2383-3688
10.22034
fa
jalali
1396
6
1
gregorian
2017
9
1
6
20
online
1
fulltext
en
کاربرد بیرونی اکسید نیتروژن
Exogenous Nitric oxide (NO) induced oxidative stress and increased production of secondary metabolites in Catharanthus roseus Callus Cells
سایر موارد
others
پژوهشي
Research
<p dir="ltr" style="text-align: justify;">ندارد<br>
Objective was to study the effect of exogenous nitric oxide on cellular response and production of secondary metabolite in Catharanthus roseus callus. The<br>
Cell suspension and callus of C. roseus was treated with sodium nitroprusside, then cell viability, morphology, H2O2 content, lipid peroxidation, proline content and the activity of superoxide dismutase, catalase and peroxidase was evaluated. Also production of total antioxidant, alkaloid, flavonoid and phenolic compounds was estimated. Significant (p<0.05) dose dependent reduction in viability was observed. In addition H2O2, Proline content, activity of the investigated enzymes and production of total antioxidant, alkaloid, flavonoid, and total phenolic compounds increased significantly. Malondialdehyde level as a lipid peroxidation indicator was also increased significantly. Sodium nitroprusside caused cellular membrane damage due to oxidative stress, but an increase in the production of secondary metabolites was observed too. Exogenous NO treatment might be a useful way to increase production of alkaloids at cellular level.</p>
<p><strong>The objective of the study was to investigate the effect of exogenous nitric oxide on cellular response and production of secondary metabolites in <em>Catharanthus roseus</em> callus. The Cell suspension and callus of <em>C. roseus</em> were treated with sodium nitroprusside, then cell viability, morphology, the amount of H<sub>2</sub>O<sub>2</sub>, proline, lipid peroxidation, the activity of superoxide dismutase, catalase and peroxidase were evaluated. Also production of total antioxidant, alkaloid, flavonoid and phenolic compounds were estimated. A Significant (p<0.05) reduction was observed in viability according to the dose of treatment. Also the amounts fo H<sub>2</sub>O<sub>2</sub>, proline, total antioxidant, alkaloid, flavonoid, and total phenolic compounds and the activity of the investigated enzymes significantly increased. Malondialdehyde level as a lipid peroxidation indicator was significantly increased in response to treatment. In conclusion we may announce that the</strong><strong> sodium nitroprusside caused cellular membrane damage due to oxidative stress, but an increase in the production of secondary metabolites was observed too. Exogenous NO treatment might be a useful way to increase production of alkaloids at cellular level.</strong></p>
کالوس, پریوش, نیتریک اکسید, تنش اکسیداتیو, متابولیتهای ثانویه
Callus, Catharanthus roseus, Nitric oxide (NO), Oxidative Stress, Secondary metabolites
49
56
http://jispp.iut.ac.ir/browse.php?a_code=A-10-638-1&slc_lang=en&sid=1
Majid
Mahdieh
مجید
مهدیه
m-mahdiyeh@araku.ac.ir
100319475328460010323
100319475328460010323
Yes
دانشگاه اراک