Volume 2, Number 5 (Vol.2, No. 5 2013)                   jppf 2013, 2(5): 49-60 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farzadnejad F, Razavizadeh R, Shabani L. Methyl isothiocyanate effect on physiological response and antioxidant enzyme activities in seedlings of Lepidium sativum L. under in vitro condition. jppf. 2013; 2 (5) :49-60
URL: http://jispp.iut.ac.ir/article-1-60-en.html

assistant professor payame noor university , razavi.roya@gmail.com
Abstract:   (6500 Views)
One of the major tissue damage following exposure to stress in plants is caused by the oxidative stress. In this study effects of different levels of methyl isothiocyanate on biochemical parameters and antioxidant enzyme activity such as ascorbate peroxidase, catalase and guaiacol peroxidase in seedling Lepidiun sativum L. under in vitro was investigated. The seeds of Lepidium sativum (Garden Cress) were sterilized and then were cultured in MS medium. After 20 days, seedlings were treated with concentrations of 0.01, 0.1 and 1 mM methyl isothiocyanate (various levels of stress) under steril condition. After 3 days, results showed chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and protein significantly decreased, while reduced sugar, proline and antioxidant enzymes increased in seedlings which were under oxidative stress. Thus methylisothiocyanate appears to cause oxidative stress and the activation parameters of the plant's defenses. The maximum and minimum, respectively, at concentrations of 1 mM and 0.01 mM methyl isothiocyanate observed.
Full-Text [PDF 229 kb]   (709 Downloads)    
Type of Study: Research | Subject: others
Received: 2013/05/6 | Accepted: 2013/07/21 | Published: 2013/11/30

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Plant Process and Function

Designed & Developed by : Yektaweb