Volume 6, Number 20 (vol. 6, no. 20, year 2017 2017)                   jppf 2017, 6(20): 17-26 | Back to browse issues page


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Hairy root induction and secondary metabolite production in Perovskia abrotanoides Karel. jppf. 2017; 6 (20) :17-26
URL: http://jispp.iut.ac.ir/article-1-636-en.html

Abstract:   (83 Views)

This study evaluated the induction of hairy roots in Perovskia abrotanoides Karel, a medicinally important member of Lamiaceae family, using different strains of Agrobacterium rhizogenes and various carbon supplies, for the first time. This research established an efficient Agrobacterium mediated transformation system for this plant species through bacterial strains including ATCC15834, TR105 and R1000. Moreover, the article investigated the influence of acetosyringone and carbon sources type on the rooting and root biomass production. The potentiality of hairy root cultures for production of tanshinones was also investigated. Hairy root induction in the nodes of intact seedlings was successful in all tested bacterial strains. According to our results, the highest frequency of transformation belonged to the nodes infected with ATCC15834 (47.33%). The strain R1000 was more effective than TR105 in hairy root induction. Transformation frequency increased up to 60.99% by the addition of 100 µM acetosyringone. The highest root dry and fresh weight per 50 mL of the nutrient medium, (0.149 and 1.996 g, respectively) and root length (34.45 cm) obtained by the strain ATCC15834 in the medium containing 3% sucrose. However, the strain R1000 induced the maximum of the lateral root density of hairy roots (7.1 branches cm-1) in the medium containing 3% glucose. PCR analysis using rolC specific primer with amplicon of 612 bp confirmed the transgenic nature of hairy roots. According to the HPLC analysis, cryptotanshinone and tanshinone IIA contents in the hairy roots induced by strain TR105 (clone T7) were 53.17±0.26 and 14.48±0.30 µg g-1 DW, respectively.

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Type of Study: Research | Subject: biotecnology
Received: 2016/05/27 | Accepted: 2016/10/1 | Published: 2017/08/27

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